Inhibition of MicroRNAs Promotes Osteogenic Differentiation Potential of Mesenchymal Stem Cells Derived from Human Placenta

Authors

Ladda Meesuk, Division of Cell Biology, Department of Preclinical Sciences, Faculty of Medicine, Thammasat University, Pathum Thani, Thailand
Jintamai Suwanprateeb, Biomedical Engineering Research Unit, National Metal and Materials Technology Center (MTEC), Ministry of Science and Technology, Pathum Thani, Thailand
Pakpoom Kheolamai, Division of Cell Biology, Department of Preclinical Sciences, Faculty of Medicine, Thammasat University, Pathum Thani, Thailand
Chairat Tantrawatpan, Division of Cell Biology, Department of Preclinical Sciences, Faculty of Medicine, Thammasat University, Pathum Thani, Thailand
Duangrat Tantikanlayaporn, Division of Cell Biology, Department of Preclinical Sciences, Faculty of Medicine, Thammasat University, Pathum Thani, Thailand
Sirikul Manochantr, Division of Cell Biology, Department of Preclinical Sciences, Faculty of Medicine, Thammasat University, Pathum Thani, Thailand

Keywords

Mesenchymal stem cell, MicroRNAs, Osteogenic differentiation, Alkaline phosphatase

Abstract

Introduction: Mesenchymal stem cells (MSCs) are multipotent stem cells that have a regenerative function. They can be classified into several cell types, including osteoblasts, adipocytes, and chondrocytes. MSCs can be isolated from the placenta with a non-invasive procedure. However, the osteogenic differentiation potential of MSCs derived from the placenta (PL-MSCs) is not very efficient. The investigation for improving the osteogenic differentiation potential of PL-MSCs is important for the application of PL-MSCs in the clinical field. MicroRNA, including miR-21, miR-27b, miR-29a, and let-7b, plays an important role as a post-transcriptional regulator in various processes. However, there is restricted data regarding the role of miRNA on osteogenic differentiation of PL-MSCs.
Objectives: This study is intended to investigate the role of miR-21, miR-27b, miR-29a, and let-7b on the osteogenic differentiation of PL-MSCs.
Methods: MSCs were isolated from the placenta using enzymatic digestion. The expressions of miRNAs during osteogenic induction were measured using quantitative real-time PCR. After transfection with anti-miRNA, the osteogenic differentiation potential was determined using alkaline phosphatase activity, alizarin red staining, and osteogenic gene expression.
Results: The results showed that miR-21, miR-27b, miR-29a, and let-7b were down-regulated during osteogenic differentiation of PL-MSCs.After the transfection with anti-miRNAs, the ALP activity was significantly increased compared with PL-MSCs cultured in an osteogenic differentiation medium without anti-miRNAs, especially on days 21 and 28. In addition, alizarin red staining and the expression of osteogenic markers were up-regulated when compared with the control group.
Conclusions: The data obtained from this study demonstrated that the inhibition of miR-21, miR-27b, miR-29a, and let-7b could enhance the osteogenic differentiation potential of MSCs derived from the placenta.

Recommended Citation

Meesuk, Ladda; Suwanprateeb, Jintamai; Kheolamai, Pakpoom; Tantrawatpan, Chairat; Tantikanlayaporn, Duangrat; and Manochantr, Sirikul. 2022 Inhibition of MicroRNAs Promotes Osteogenic Differentiation Potential of Mesenchymal Stem Cells Derived from Human Placenta. Asian Medical Journal and Alternative Medicine. 22, 4 (Aug. 2022 ), Meesuk, L. , Suwanprateeb, J. , Kheolamai, P. , Tantrawatpan, C. , Tantikanlayaporn, D. and Manochantr, S. 2022. Inhibition of MicroRNAs Promotes Osteogenic Differentiation Potential of Mesenchymal Stem Cells Derived from Human Placenta. Asian Medical Journal and Alternative Medicine. 22, - (Aug. 2022), S139. DOI:https://doi.org/10.14456/2022s10746 .-.
Available at: https://doi.org/10.70933/2773-9465.1262

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