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Onmanee Prajuabjinda, Department of Applied Thai Traditional Medicine, Faculty of Medicine, Thammasat University, Pathum Thani, Thailand
Bhanuz Dechayont, Department of Applied Thai Traditional Medicine, Faculty of Medicine, Thammasat University, Pathum Thani, Thailand
Jitpisute Chunthorng-Orn, Department of Applied Thai Traditional Medicine, Faculty of Medicine, Thammasat University, Pathum Thani, Thailand
Pathompong Phuaklee, Department of Applied Thai Traditional Medicine, Faculty of Medicine, Thammasat University, Pathum Thani, Thailand
Nuttika Prommee, Division of Applied Thai Traditional Medicine, Faculty of Public Health, Naresuan University, Phitsanulok, Thailand
Saovapak Poomirat, Department of Applied Thai Traditional Medicine, Faculty of Medicine, Thammasat University, Pathum Thani, Thailand
Maneewan Phanthong, Department of Applied Thai Traditional Medicine, Faculty of Medicine, Thammasat University, Pathum Thani, Thailand
Pannawat Chaiyawatthanananthn, Department of Applied Thai Traditional Medicine, Faculty of Medicine, Thammasat University, Pathum Thani, Thailand
Arunporn Itharat, Department of Applied Thai Traditional Medicine, Faculty of Medicine, Thammasat University, Pathum Thani, Thailand
Kitrawee Jiraratsatit, Department of Innovative Health Promotion, Faculty of Public Health, Thammasat University, Pathum Thani, Thailand
Kitiya Yangthaworn, Department of Applied Thai Traditional Medicine, Faculty of Medicine, Thammasat University, Pathum Thani, Thailand

Keywords

Ayuwattana Wayuvek remedy, Cytotocix, Antioxidant

Abstract

Introduction: An incidence of breast cancer is the highest over the decade. Breast cancer is the most common cancer in women specifically in developing country, including Thailand. Medicinal plants have been used for medicinal purpose and treatment cancer patients. Ayuwattana Wayuvek (AW) remedy is used for balancing an element of body to preventing cancer or any disease. However, there are no report to confirm the effective of this remedy.
Objectives: This research was to investigate cytotoxic activity and determine antioxidant activities of AW remedy and its components extracts.
Methods: Plant materials were divided to two parts. An ethanolic extract was macerated in 95% ethanol, and an aqueous extract was boiled in water. All of extracts were to investigate cytotoxic activity on MCF-7 cell by SRB assay and determine antioxidant activities by DPPH assay.
Results: In cytotoxic activity, the results exhibited that only the ethanolic extracts against on MCF-7. The ethanolic extract of M. Minutum had highest cytotoxic against with IC50 2.78 ± 0.17 ug/mL. Moreover, the ethanolic extracts of AW remedy had cytotoxic against with IC50 7.18 ± 0.28 ug/mL. In antioxidant activity, the ethanolic extract of C.bejolghota displayed the highest activity with IC50 3.79 ± 0.37 ug/mL compared to 13.95 ± 1.06 ug/mL (BHT), and both of the ethanolic and aqueous extracts ofAW remedy inhibited antioxidant activity with IC50 9.79 ± 0.37 ug/mL and 9.04 ± 3.42 ug/mL, respectively.
Conclusions: Ourstudy revealed that the ethanolic extract displayed a promising cytotoxic effect on breast cancer cell lines. However, these data should be more studied on molecular biology in the future.

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