Human Placenta-derived Mesenchymal Stem Cells Inhibit Proliferation and Promote Apoptosis in Cholangiocarcinoma Cell Lines via Suppressing JAK2/STAT3 Signaling

Authors

Tanachapa Jantalika, Division of Cell Biology, Department of Preclinical Sciences, Faculty of Medicine, Thammasat University, Pathum Thani, Thailand and Center of Excellence in Stem Cell Research, Thammasat University, Pathum Thani, Thailand
Chairat Tantrawatpan, Division of Cell Biology, Department of Preclinical Sciences, Faculty of Medicine, Thammasat University, Pathum Thani, Thailand and Center of Excellence in Stem Cell Research, Thammasat University, Pathum Thani, Thailand
Sirikul Manochantr, Division of Cell Biology, Department of Preclinical Sciences, Faculty of Medicine, Thammasat University, Pathum Thani, Thailand and Center of Excellence in Stem Cell Research, Thammasat University, Pathum Thani, Thailand
Duangrat Tantikanlayaporn, Division of Cell Biology, Department of Preclinical Sciences, Faculty of Medicine, Thammasat University, Pathum Thani, Thailand and Center of Excellence in Stem Cell Research, Thammasat University, Pathum Thani, Thailand
Pakpoom Kheolamai, Division of Cell Biology, Department of Preclinical Sciences, Faculty of Medicine, Thammasat University, Pathum Thani, Thailand and Center of Excellence in Stem Cell Research, Thammasat University, Pathum Thani, Thailand

Keywords

Apoptosis, Cholangiocarcinoma, Conditioned media, Mesenchymal stem cells, Placenta

Abstract

Introduction: Cholangiocarcinoma (CCA) is an aggressive malignancy originating from the bile duct. Incidence and mortality increase worldwide because of late diagnosis and a lack of therapeutic options. Mesenchymal stem cells (MSC) can secrete several cytokines to promote or inhibit the progression of many cancer cell types. However, the effect of human placenta-derived MSCs (PL-MSC) on CCA progression has not been clarified.

Objectives: The present study aims to investigate the effects and molecular mechanisms of conditioned media derived from PL-MSC (PL-CM) on three CCA cell lines (KKU100, KKU213A, and KKU213B).

Methods: CCA cells were cultured with PL-CM to examine cell proliferation and apoptosis by MTT assay and annexin V/PI analysis, respectively. JC-1 staining, caspase 3 activity, and western blot were used to investigate the mechanism of PL-CM induced apoptosis of CCA cell lines through mitochondrial apoptotic pathway and JAK2/STAT3 signaling.

Results: PL-CM could suppress proliferation and promote apoptosis of all CCA cell lines by inducing mitochondrial apoptotic pathways, most likely through suppression of the JAK2/ STAT3 signaling pathway.

Conclusions: This study suggests PL-CM has a potent anti-cancer effect on CCA cells. Therefore, PL-MSC may provide a basis for further clinical trials to study as well as a possible alternative treatment for CCA patients.

Recommended Citation

Jantalika, Tanachapa; Tantrawatpan, Chairat; Manochantr, Sirikul; Tantikanlayaporn, Duangrat; and Kheolamai, Pakpoom. 2022 Human Placenta-derived Mesenchymal Stem Cells Inhibit Proliferation and Promote Apoptosis in Cholangiocarcinoma Cell Lines via Suppressing JAK2/STAT3 Signaling. Asian Medical Journal and Alternative Medicine. 22, 4 (Aug. 2022 ), Jantalika, T., Tantrawatpan, C., Manochantr, S., Tantikanlayaporn, D. and Kheolamai, P. 2022. Human Placenta-derived Mesenchymal Stem Cells Inhibit Proliferation and Promote Apoptosis in Cholangiocarcinoma Cell Lines via Suppressing JAK2/STAT3 Signaling. Asian Medical Journal and Alternative Medicine. 22, - (Aug. 2022), S111. DOI:https://doi.org/10.14456/2022s10720.-.
Available at: https://doi.org/10.70933/2773-9465.1236

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