The Expression of Alkaline Phosphatase in Metformin-treated Umbilical Cord-derived Mesenchymal Stem Cells

Authors

Nuengruethai Chadee, Division of Cell Biology, Department of Preclinical Sciences, Faculty of Medicine, Thammasat University, Pathum Thani, Thailand
Jintamai Suwanprateep, Biomedical Engineering Research Unit, National Metal and Materials Technology Center (MTEC), Ministry of Science and Technology, Pathum Thani, Thailand
Chairat Tantrawatpan, Division of Cell Biology, Department of Preclinical Sciences, Faculty of Medicine, Thammasat University, Pathum Thani, Thailand
Pakpoom Kheolamai, Division of Cell Biology, Department of Preclinical Sciences, Faculty of Medicine, Thammasat University, Pathum Thani, Thailand
Duangrat Tantikanlayaporn, Division of Cell Biology, Department of Preclinical Sciences, Faculty of Medicine, Thammasat University, Pathum Thani, Thailand
Sirikul Manochantr, Division of Cell Biology, Department of Preclinical Sciences, Faculty of Medicine, Thammasat University, Pathum Thani, Thailand

Keywords

Mesenchymal stem cells, Metformin, Osteogenic differentiation, Umbilical cord, Alkaline phosphatase

Abstract

Introduction: Mesenchymal stem cells (MSCs) have gained more interest in regenerative medicine. The umbilical cord, the alternative source of MSCs, has thus been increasingly recognized as a good MSC source for bone tissue regeneration and bone defect repair. Metformin, an anti-hyperglycemic drug, has been reported that promotes the osteogenic differentiation of BM-MSCs.
Objectives: This study aims to investigate the viability and the expression of alkaline phosphatasein metformin-treated umbilical cord-derived MSCs (UC-MSCs) during osteogenicdifferentiation.
Methods: The UC-MSCs were obtained from pregnant women after normal delivery. After charac-terization, UC-MSCs were cultured with different metformin concentrations (0 - 160 μM). The cell viability and alkaline phosphatase (ALP) expression were observed by MTT and alkaline phosphatase activity assay.
Results: The result showed that metformin at a concentration of 0 - 160 μM did not decrease the viability of UC-MSCs. Interestingly, the alkaline phosphatase activity of metformin-treated UC-MSCs was significantly increased, compared with untreated UC-MSCs in a dose-dependent manner.
Conclusions: The data suggested that metformin could enhance the expression of alkaline phosphatase and the osteogenic differentiation ability of MSCs derived from the umbilical cord.

Recommended Citation

Chadee, Nuengruethai; Suwanprateep, Jintamai; Tantrawatpan, Chairat; Kheolamai, Pakpoom; Tantikanlayaporn, Duangrat; and Manochantr, Sirikul. 2022 The Expression of Alkaline Phosphatase in Metformin-treated Umbilical Cord-derived Mesenchymal Stem Cells. Asian Medical Journal and Alternative Medicine. 22, 4 (Aug. 2022 ), Chadee, N. , Suwanprateep, J. , Tantrawatpan, C. , Kheolamai, P. , Tantikanlayaporn, D. and Manochantr, S. 2022. The Expression of Alkaline Phosphatase in Metformin-treated Umbilical Cord-derived Mesenchymal Stem Cells. Asian Medical Journal and Alternative Medicine. 22, - (Aug. 2022), S110. DOI:https://doi.org/10.14456/2022s10719.-.
Available at: https://doi.org/10.70933/2773-9465.1235

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