Validation of HPLC Method for the Determination of Anti-allergic Compounds in Ethanolic Extract of Prasaprohyai remedy, a Thai Traditional Medicine

Authors

Nichamon Mukkasombut, Department of Applied Thai Traditional Medicine, Faculty of Medicine, Thammasat University, Pathumthani, 12120 Thailand
Weerachai Pipatrattanaseree, Regional Medical Science Center 12 Songkhla, Department of Medical Sciences, Songkhla, 90100, Thailand
Arunporn Itharat, Department of Applied Thai Traditional Medicine, Faculty of Medicine, Thammasat University, Pathumthani, 12120 Thailand. and Center of Excellence in Applied Thai Traditional Medicine Research, Thammasat University, Pathumthani, 12120, Thailand

Keywords

HPLC, Quality control, Prasaprohyai remedy, Validated method, Ethyl-p-methoxy- cinnamate, Eugenol

Abstract

Introduction: Prasaprohyai remedy (PY) is a Thai traditional medicine that has long been used to treat the common cold and fever. Previous studies revealed that 3,000 mg/day of powdered PY capsule were able to relieve allergic rhinitis symptoms as effectively as 10 mg/day of Loratadine. PY consists of 21 herbs and there is a need for quality control to ascertain their standard before being used in patients. Earlier studies showed that Ethyl-p-methoxycinnamate (EPMC) and eugenol had the potential as markers for quality control of PY.

Objective: To develop a validated method for Prasaprohyai remedy extract.

Methods: HPLC methodology was performed to assess validation parameters.

Results: The validation parameters addressed were specificity, limit of detection, limit of quantification, linearity, accuracy and precision. The developed HPLC system was specific for the detection of EPMC and eugenol content in Prasaprohyai remedy. All other parameters for analysis complied with standard requirements. For EPMC the analytical range was 25-450 µg/ml with linearity (r²=0.9999), LOD 0.1 µg/ml, LOQ 0.5 µg/ml. The analytical range of eugenol was 2.5-30 µg/ml with linearity (r²= 0.9998), LOD 0.25 µg/ml, LOQ 0.5 µg/ml.

Conclusion: The developed HPLC method can be used to standardize the Prasaprohyai remedy extract.

Recommended Citation

Mukkasombut, Nichamon; Pipatrattanaseree, Weerachai; and Itharat, Arunporn. 2020 Validation of HPLC Method for the Determination of Anti-allergic Compounds in Ethanolic Extract of Prasaprohyai remedy, a Thai Traditional Medicine. Asian Medical Journal and Alternative Medicine. 20, 1 (Jan. 2020 ),
Available at: https://doi.org/10.70933/2773-9465.1687

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